Chow Yoke Yue, Zariyantey Abd Hamid, Enoch Kumar Perimal, Penny George, B. Hemabarathy Bharatham


Apatite layers formed by simulated body fluid (SBF) on the surface of calcium-based scaffolds have been proven to enhance the osteoblastic activity of pre-osteoblasts and osteogenic activity of bone marrow-derived stem cell (BM-SCs). Previously developed Alginate/Cockle shell powder nanobiocomposite bone scaffold (Alg/nCP) has been shown to possess excellent osteoconductive properties. The effect of pre-mineralization of the scaffold surface towards the growth and differentiation of BM-SCs’ were evaluated using microscopic and biochemical methods in scaffolds divided into SBF pre-treated and control groups at two time points. MTT proliferation assay showed statistically significant decrease in cell proliferation in SBF group for both culture periods. SEM observation revealed growth of BM-SCs and scaffold surface mineralisation and calcium deposition in both groups with higher intensity observable in the control group. Supporting biochemical studies showed a significant decrease in alkaline phosphatase (ALP) level indicating a lesser osteogenic differentiation in the SBF group as compared to control. Pre-mineralisation of scaffolds in SBF produced a contradicting result in which it did not provide a better environment for growth and proliferation of BM-SCs. However, the Alg/nCP scaffold did show potentials in supporting the osteogenic differentiation of the stem cells.


Nanobiocomposite bone scaffold, simulated body fluid, bone marrow-derived stem cell, mineralisation, osteogenic differentiation

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Meng, X., Leslie, P., Zhang, Y. & Dong, J. 2014. Stem Cells in a Three-dimensional Scaffold Environment. Springerplus. 3(1): 80.

Shafiu Kamba, A. & Zakaria, Z. a. B. 2014. Osteoblasts Growth Behaviour on Bio-Based Calcium Carbonate Aragonite Nanocrystal. BioMed Research International. 2014.

Zhang, Y., L. Xia, D. Zhai, M. Shi, Y. Luo, C. Feng, B. Fang, J. Yin, J. Chang, C. Wu (2015) Mesoporous Bioactive Glass Nanolayer-functionalized 3D-printed Scaffolds for Accelerating Osteogenesis and Angiogenesis. Nanoscale. 7: 19207-19221.

Whited, B. M., Whitney, J. R., Hofmann, M. C., Xu, Y. & Rylander, M. N. 2011. Pre-osteoblast Infiltration and Differentiation in Highly Porous Apatite-coated Plla Electrospun Scaffolds. Biomaterials. 32(9): 2294-2304.

Miguel, B. S., Kriauciunas, R., Tosatti, S., Ehrbar, M., Ghayor, C., Textor, M. & Weber, F. E. 2010. Enhanced Osteoblastic Activity and Bone Regeneration Using Surface-Modified Porous Bioactive Glass Scaffolds. Journal of Biomedical Materials Research Part A. 94A(4): 1023-1033.

Bharatham, B. H., Bakar, A., Zuki, M., Perimal, E. K., Yusof, L. M. & Hamid, M. 2014. Development and Characterization of Novel Porous 3d Alginate-cockle Shell Powder Nanobiocomposite Bone Scaffold. BioMed Research International. 2014: 146723-11. DOI:

Bernhardt, A., Despang, F., Lode, A., Demmler, A., Hanke, T. & Gelinsky, M. 2009. Proliferation and Osteogenic Differentiation of Human Bone Marrow Stromal Cells on Alginate–Gelatine–Hydroxyapatite Scaffolds with Anisotropic Pore Structure. Journal of Tissue Engineering and Regenerative Medicine. 3(1): 54-62.

Kim, J.-J., Jin, G.-Z., Yu, H.-S., Choi, S.-J., Kim, H.-W. & Wall, I. B. 2012. Providing Osteogenesis Conditions to Mesenchymal Stem Cells Using Bioactive Nanocomposite Bone Scaffolds. Materials Science and Engineering: C. 32(8): 2545-2551.

Eslaminejad, M. B. & Faghihi, F. 2011. Mesenchymal Stem Cell-Based Bone Engineering for Bone Regeneration. Dlm. Eberli, D. (pnyt.). Regenerative Medicine and Tissue Engineering - Cells and Biomaterials. Ch. 03. Rijeka: InTech.

Giuliani, N., G. Lisignoli, M. Magnani, C. Racano, M. Bolzoni, B. Dalla Palma, A. Spolzino, C. Manferdini, C. Abati, D. Toscani, A. Facchini, F. Aversa. 2013. New Insights Into Osteogenic And Chondrogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells and Their Potential Clinical Applications for Bone Regeneration in Pediatric Orthopaedics. Stem Cells Int. 2013: 312501.

Oryan, A., Kamali, A., Moshiri, A., Baghaban Eslaminejad, M. 2017. Regenerative Medicine: What is the Evidence? Cells Tissues Organs. 204: 59-83. DOI:

Mavis, B., Demirtaş, T. T., Gümüşderelioğlu, M., Gündüz, G. & Çolak, Ü. 2009. Synthesis, Characterization and Osteoblastic Activity of Polycaprolactone Nanofibers Coated with Biomimetic Calcium Phosphate. Acta Biomaterialia. 5(8): 3098-3111.

Tas, A. C. & Bhaduri, S. B. 2011. Rapid Coating of Ti6al4v at Room Temperature with a Calcium Phosphate Solution Similar to 10× Simulated Body Fluid. Journal of Materials Research. 19(9): 2742-2749.

González-Vázquez, A., Planell, J. A. & Engel, E. 2014. Extracellular Calcium and Casr Drive Osteoinduction in Mesenchymal Stromal Cells. Acta Biomaterialia. 10(6): 2824-2833.

Salzig, D., Leber, J., Merkewitz, K., Lange, M. C., Ster, N. & Czermak, P. 2016. Attachment, Growth, and Detachment of Human Mesenchymal Stem Cells in a Chemically Defined Medium. Stem Cells International. 2016(10). DOI:

Ren. T., Ren, J., Jia, X., Pan, K. 2005. The Bone Formation in Vitro and Mandibular Defect Repair Using PLGA Porous Scaffolds. Journal of Biomedical Materials Research Part A. 74A(4): 562-569.

Lai, G.-J., Shalumon, K. T. & Chen, J.-P. 2015. Response of Human Mesenchymal Stem Cells to Intrafibrillar Nanohydroxyapatite Content and Extrafibrillar Nanohydroxyapatite in Biomimetic Chitosan/Silk Fibroin/Nanohydroxyapatite Nanofibrous Membrane Scaffolds. International Journal of Nanomedicine. 10(567-584).

Oh, S.-A., Kim, S.-H., Won, J.-E., Kim, J.-J., Shin, U. S. & Kim, H.-W. 2010. Effects on Growth and Osteogenic Differentiation of Mesenchymal Stem Cells by the Zinc-Added Sol-Gel Bioactive Glass Granules. Journal of Tissue Engineering. 1(1): 475260.

Abou Neel, E. A., Chrzanowski, W., Georgiou, G., Dalby, M. J. & Knowles, J. C. 2010. In Vitro Biocompatibility and Mechanical Performance of Titanium Doped High Calcium Oxide Metaphosphate-Based Glasses. Journal of Tissue Engineering. 1(1): 390127.

Seyedjafari, E., Soleimani, M., Ghaemi, N., Sarbolouki, M. N. 2011. Enhanced Osteogenic Differentiation of Cord Blood-derived Unrestricted Somatic Stem Cells on Electrospun Nanofibers. Journal of Materials Science: Materials in Medicine. 22: 165-74.

Maleki, H., Shahbazi, M. A., Montes, S., Hosseini, S. H., Eskandari, M. R., Zaunschirm, S., Verwanger, T., Mathur, S., Milow, B., Krammer, B. & Husing, N. 2019. Mechanically Strong Silica-silk Fibroin Bioaerogel: A Hybrid Scaffold with Ordered Honeycomb Micromorphology and Multiscale Porosity for Bone Regeneration. ACS Appl Mater Interfaces.

Zhu, D.-Y., Lu, B., Yin, J.-H., Ke, Q.-F., Xu, H., Zhang, C.-Q., Guo, Y.-P. & Gao, Y.-S. 2019. Gadolinium-doped Bioglass Scaffolds Promote Osteogenic Differentiation of Hbmsc Via the Akt/Gsk3β Pathway and Facilitate Bone Repair in Vivo. International Journal of Nanomedicine. 14(1085-1100.

Yassin, M. A., Fuoco, T., Mohamed-Ahmed, S., Mustafa, K. & Finne-Wistrand, A. 2019. 3d and Porous Rgdc-Functionalized Polyester-based Scaffolds as a Niche to Induce Osteogenic Differentiation of Human Bone Marrow Stem Cells. Macromol Biosci. e1900049.



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