VOLATOLOMICS COMBINED TERAHERTZ TIME - DOMAIN SPECTRAL ANALYSES OF COLON CANCER IN VITRO

Ahmad Zulhilmi Arshad, Yusof Munajat, Sib Krishna Ghoshal, Rahman Jamal, Nor Adzimah Johdi, Raja Kamarulzaman Raja Ibrahim, Hanif Zulkhairi

Abstract


Terahertz time-domain spectroscopy (THz-TDS) offers a great advantage for the analysis of biological samples. In this study, we combined THz-TDS with volatolomics analysis and analyzed 2 colon cell lines via in vitro settings. The release of volatile organic compounds (VOCs) was measured from the normal colon (CCD112CoN) and cancer colon (COLO320DM) cell lines which were grown in sealed flasks. Data validation were carried out with principal component analysis (PCA) and partial least square (PLS) scores while the chemometric analyses were performed using Camo Unscrambler X software. In-depth THz-TDS spectral analysis of the cancer colon (COLO320DM) cell line shows significant traces of benzamide gas when validated using gas chromatography-mass selective detection (GC-MSD) system. This preliminary data shows the potential use of identification and quantification of benzamide compound in the cancer colon cells and this could provide useful insight towards cancer drug design and therapy.


Keywords


Volatolomics analysis, colon cancer, Chromatography, Terahertz spectra, gas analysis

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DOI: https://doi.org/10.11113/jt.v81.13310

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